Potter Elvehjem Homogenizer Vs Dounce Homogenizer. PDF filePotter S Homogenizer For gentle cell disruption The Potter S homogenizer of Sartorius is a wellestablished tool in laboratories worldwide where gentle disruption of soft tissues or cultured cells is required Due to its wellcontrolled action this homogenizer can even be used for isolation of nuclei from such materials.
We offer a range of handheld grinders from DWK KIMBLE ® Dounce AllGlass tissue grinders designed for cellular work in which the nucleus remains intact after homogenization to Benchmark Scientific′s handheld rotorstator homogenizer the D1000 This works by drawing the sample in liquid into the generator probe with the variable speed rotor and then forcing it out.
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Cell Disruption Sonication vs Homogenization The preparation of samples for protein and nucleic acid analysis requires two essential steps disruption of the tissue to release individual cells and lysis of those cells to release their cellular contents Mechanical homogenization and sonication (sometimes referred to as ultrasonic.
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PotterElvehjem Homogenizer A PotterElvehjem homogenizer is made up on a pestle – either manually or mechanically driven – that is driven into a rounded vessel Similar to the Dounce homogenizer the PotterElvehjem homogenizer manually disrupts and lyses cells its efficacy and efficiency is determined by the speed at which the strokes are administered by the user.
Two glass homogenizers used for shearing cells. The Dounce
The Dounce homogenizer (left) is manually operated and disrupts cells by forcing them between the pestle and tube wall The PotterElvehjem homogenizer can be hand operated or attached to a motor The pestle can be PTFE or glass Figure 11 Though the homogenate appears very fine significant tissue was left intact.
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Potter S Homogenizer For gentle cell disruption
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PDF fileDounce homogenizer Alternatively as described by Frezza et al (2007) resuspend the cell pellet in 9 mL of icecold RSB hypo buffer and transfer 3 mL of the cells at a time to a 5mL Potter–Elvehjem homogenizer with a Teflon pestle 2 Allow the cells to swell for 5–10 min Check the progress of the swelling using a phasecontrast microscope 3.